We narrowed to 19,852 results for: Nov;
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Plasmid#63851PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromoterAEB3Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICSL11061
Plasmid#68255PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-1
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-F-Gcn5-NLSm
Plasmid#63708Purposeretroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cellsDepositorInsertGcn5 (Kat2a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationmutated K425, K427 and K438 to R (arginine)Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
TagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(K433E)
Plasmid#42514DepositorInsertPyruvate Kinase M2 Lys433->Glu
UseRetroviralTagsFlagMutationChanged lysine 433 to glutamateAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDuex2-rsGreenF-E
Plasmid#205752PurposeExpression of rsGreenF-E in mamalian cellsDepositorInsertrsGreenF-E
ExpressionMammalianMutationwtAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDuex2-rsEGFP2-E
Plasmid#205753PurposeExpression of rsEGFP2-E in mamalian cellsDepositorInsertrsEGFP2-E
ExpressionMammalianMutationwtAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRec ΔLacI
Plasmid#104575PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.DepositorInsertPTet-cas12, tetR
UseSynthetic BiologyAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP
Plasmid#103997PurposePlasmid encoding TagRFPDepositorInsertTagRFP
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mClover3
Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxVenus
Plasmid#103993PurposePlasmid encoding moxVenusDepositorInsertmoxVenus
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEmerald
Plasmid#103982PurposePlasmid encoding mEmeraldDepositorInsertmEmerald
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-VPH2 chr I
Plasmid#41897PurposeYeast expression of VPH2.DepositorAvailable SinceFeb. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
stabilized HP35 tension sensor module
Plasmid#101251PurposeExpresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.DepositorInsertYPet(short)-HP35st-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AMPH1-BAR-mCherry
Plasmid#85130PurposeBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherryDepositorInsertBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
TagsmCherryExpressionMammalianPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only