Addgene

We narrowed to 2,432 results for: gem

Showing: 461 - 470 of 2432 results

Results per page:

Sort results by:

pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

Plasmid

#169338

Purpose

Expression of the LT-Fucci(CA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(CA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

Plasmid

#202644

Purpose

Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

Depositor

Balázs Enyedi

Article

Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

Insert

PM-mKate2-P2A-GEM-LTB4mut

Use

Tags

PM-mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

Plasmid

#223959

Purpose

Dual fluorescent reporter for histone H2B and for APC/C activity

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert

Plasmid

#111153

Purpose

knock in bait for Axolotl Pax7 locus

Depositor

Elly Tanaka

Article

Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

Insert

GAP43-Cherry-T2a-mERt-Cre-Ert

Use

Cre/Lox

Tags

Expression

Mutation

wt

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

Plasmid

#225441

Purpose

Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

Depositor

Diego Orzaez

Insert

hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

Plasmid

#193139

Purpose

Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)

Use

Lentiviral

Tags

Expression

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

EF1a

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge