Addgene

We narrowed to 3,733 results for: atr;

Showing: 921 - 940 of 3733 results

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MS2_adRNA-MCP_ADAR1 DD_NLS

Plasmid

#124625

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0112

Plasmid

#117548

Purpose

Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0113

Plasmid

#117552

Purpose

Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-GAC site)

Plasmid

#170149

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(GAC)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (RAB7A-GAC site)

Plasmid

#170140

Purpose

AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-RAB7A(GAC)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV cleavable His10

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[WIV1 RBD]

Plasmid

#190087

Purpose

express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta2 RBD]

Plasmid

#190083

Purpose

express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

Plasmid

#190082

Purpose

express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

Plasmid

#190081

Purpose

express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

Plasmid

#190085

Purpose

express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0899

Plasmid

#177092

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0895

Plasmid

#177091

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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