We narrowed to 1,262 results for: grna cloning vector

1. Sha3Cas9

   Plasmid

   #192136

   Purpose

   Expresses Sha3Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

   Insert

   Sha3Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-BbsI-GGsite-mCherry-Puro

   Plasmid

   #221232

   Purpose

   Cloning vector for sgRNA with mCherry and Puro

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-Sa-SauriCas9

   Plasmid

   #135967

   Purpose

   Expresses Sa-SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sha2Cas9

   Plasmid

   #192140

   Purpose

   Expresses Sha2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   Sha2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpeCas9

   Plasmid

   #192139

   Purpose

   Expresses SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

   Plasmid

   #192132

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-Y
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (Y446A)

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 CARD8 PAM

   Plasmid

   #169975

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   PAM

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

   Plasmid

   #241191

   Purpose

   Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

   Depositor

   Junsu Kang

   Insert

   3x-FLAG; mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

    Plasmid

    #241190

    Purpose

    Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

    Depositor

    Junsu Kang

    Insert

    V5-APEX2n; mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIREN-RetroQ-shLuc

    Plasmid

    #73666

    Purpose

    pSRIEN-RetroQ vector containing control sequence luciferase

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTGC-U6.2

    Plasmid

    #112809

    Purpose

    PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Insert

    U6:2-gRNAcore
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTGC-U6.3

    Plasmid

    #112810

    Purpose

    PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Insert

    U6:3-gRNAcore
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p944-SWITCH-OVER insert: EF1a-Puro

    Plasmid

    #217891

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p931-SWITCH-OVER insert: EF1a-Blasti

    Plasmid

    #217890

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid

    #124299

    Purpose

    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    UBE2G1 (UBE2G1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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