We narrowed to 1,335 results for: piggyBac

1. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

   Plasmid

   #129537

   Purpose

   PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   tetR-3xFlag-HA-2A-Puro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH231-EF1-GFP-HYGRO

   Plasmid

   #115144

   Purpose

   Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH231-EF1-RFP-HYGRO

   Plasmid

   #115145

   Purpose

   Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKH151_PB_flag-LC3B_wt_IRES-BFP

   Plasmid

   #214976

   Purpose

   Flag tagged LC3B

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   FLAG-LC3B (MAP1LC3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster vasa

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-REVERBA-mScarlet-NLS-PEST

   Plasmid

   #190658

   Purpose

   Fluorescent reporter for circadian rhythms

   Depositor

   Mary Teruel

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

   Insert

   Reverba-mScarlet (Nr1d1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

   Plasmid

   #214977

   Purpose

   Flag tagged LDS mutant LC3B

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   FLAG-LC3B_mut (MAP1LC3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LDS mutant

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Promoter

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL005635 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC150-KEIMA-REEP5

    Plasmid

    #201928

    Purpose

    PiggyBac Vector expressing KEIMA-REEP5

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid

    #191154

    Purpose

    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP-t70587
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC150-TEX264-GFP

    Plasmid

    #201931

    Purpose

    PiggyBac Vector expressing TEX264-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-EF1-IRES-NEO deleted ITR

    Plasmid

    #67906

    Purpose

    PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC150-RFP-EGFP-LGALS3 R186S

    Plasmid

    #175779

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R186S

    Promoter

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH231-EF1-Puro

    Plasmid

    #115143

    Purpose

    Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Puromycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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