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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,356 results for: lat

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Showing: 9421 - 9440 of 36356 results

  2. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

    Plasmid
    #175554
    Purpose
    Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NANOG
    Use
    Mouse Targeting
    Tags
    FKBPV-HA2-P2A-eGFP
    Expression
    Mammalian
    Mutation
    none
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175554-plasmid-map-sequence-id-345080

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  3. pCS2+-Pard3-EGFP-PIF6

    Plasmid
    #154914
    Purpose
    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).
    Depositor
    Clare Buckley, Jonathan Clarke
    Publication
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    Use
    Xenopus/avian/zebrafish
    Tags
    1-100 amino acids of Arabidopsis PIF6 and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154914-plasmid-map-sequence-id-308721

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  4. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  5. FKBP-beta3(702-1094)-GFP

    Plasmid
    #100734
    Purpose
    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis
    Depositor
    Stephen Royle
    Publication
    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AP3B1 (AP3B1 Human)
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    appendage and ear only
    Promoter
    CMV
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100734-plasmid-map-sequence-id-193982

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  7. p-mCherry-C1-Phobos_CA

    Plasmid
    #98169
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct Phobos_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98169-plasmid-map-sequence-id-182726

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  8. G-GECO1.2-Orai1Y80E

    Plasmid
    #73565
    Purpose
    Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx
    Depositor
    Michael Cahalan
    Publication
    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)
    Tags
    G-GECO1.2
    Expression
    Mammalian
    Mutation
    changed tyrosine 80 of Orai1 to glutamate
    Promoter
    CMV Immediate Early
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73565-plasmid-map-sequence-id-136822

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  9. P41-bio

    Plasmid
    #47739
    Purpose
    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimised P41
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47739-plasmid-map-sequence-id-68748

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  15. SPN-bio-His

    Plasmid
    #51829
    Purpose
    Expresses full-length Leukosialin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SPN (SPN Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51829-plasmid-map-sequence-id-79665

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Showing: 9421 - 9440 of 36356 results