We narrowed to 11,622 results for: VARS

1. pDB007ns2a

   Plasmid

   #99214

   Purpose

   Accessory plasmid for PACE of PylRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
   

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and Ptet

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB038a

   Plasmid

   #99211

   Purpose

   Accessory plasmid for PACE of PylRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 29 and 184 of gIII

   Promoter

   Ppsp and PproK

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Paxillin DN

   Plasmid

   #186153

   Purpose

   For expressing paxillin variants in mammalian cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Paxilln residues 1-321 added to N-terminus of WT …

   Promoter

   cmv

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-Gncp-iGluSnFR

   Plasmid

   #107337

   Purpose

   Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

   Insert

   GltI based and noncircularly permutated glutamate biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYC56

   Plasmid

   #63910

   Purpose

   Recipient integrative vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB038b

   Plasmid

   #99212

   Purpose

   Accessory plasmid for PACE of PylRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 29, 83, and 184 of gIII

   Promoter

   Ppsp and PproK

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL2_176

   Plasmid

   #199108

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL2_169

   Plasmid

   #199101

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-mito-CitroffRH

   Plasmid

   #158548

   Purpose

   Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   CitroffRH
   

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mFGF8a

    Plasmid

    #22088

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pSC101-GFPmut2-mScarlet-I TGATC

    Plasmid

    #208190

    Purpose

    nonsense mistranslation, TGA stop variant 2

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TGA*(stop) 7TCT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL2_173

    Plasmid

    #199105

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. peCALWY-NB

    Plasmid

    #22239

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY non-binding variant
    

    Expression

    Mammalian

    Mutation

    C284S, C287S, C413S and C416S mutations linker c…

    Available Since

    Jan. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEYFP-N1-HsSmg7_H

    Plasmid

    #146476

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg7 (SMG7 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation G2560A (V854I) and three …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2_168

    Plasmid

    #199100

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBT-RP2-1

    Plasmid

    #31828

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Promoter

    beta Actin

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. cpLOV2-V5

    Plasmid

    #168991

    Purpose

    circularly permuted LOV2 variant-V5

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    cpLOV2-V5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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