We narrowed to 33,014 results for: REP

Showing: 9441 - 9460 of 33014 results

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pITEu2

Plasmid

#218282

Purpose

Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJE13HD7

Plasmid

#218281

Purpose

Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR-PMG (pXK-1650)

Plasmid

#208858

Purpose

HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Article

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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1114H

Plasmid

#200640

Purpose

An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

Depositor

Omar Akbari

Article

Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

Insert

gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.

Use

CRISPR

Expression

Insect

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192399

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192398

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

Plasmid

#192373

Purpose

To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

Plasmid

#192376

Purpose

To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-uORF-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

Plasmid

#192375

Purpose

To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-35Ster-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192401

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192400

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192402

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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