We narrowed to 66,587 results for: des.1

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

Plasmid

#224779

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

Plasmid

#224778

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

Plasmid

#224776

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / S562C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-S131C)

Plasmid

#224759

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S562C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-wt)

Plasmid

#224758

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRevA-TEV-His12

Plasmid

#137034

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAF07416.1 (revA Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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MPS-1

Plasmid

#113057

Purpose

Expresses worm MPS-1 in mammalian cells

Depositor

Federico Sesti

Article

Bianchi et al J Biol Chem. 2003 Apr 4;278(14):12415-24

Insert

MiRP K+ channel accessory Subunit 1 1 (mps-1 Nematode)

Expression

Mammalian

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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FLT1 gRNA (BRDN0001146782)

Plasmid

#75558

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-PSD-95 [K28/43R]

Plasmid

#128618

Purpose

Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-BetaActin-SecA5-YFP

Plasmid

#105664

Purpose

Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

Depositor

Qing Deng

Article

Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

Insert

Secreted Annexin5-EYFP (ANXA5 Human)

Use

Tol2-gateway

Tags

EYFP

Promoter

Beta-Actin

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS (L4-R1)

Plasmid

#61374

Purpose

5' Gateway Entry Vector Containing QUAS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QUAS

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Bacterial

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-HA

Plasmid

#27765

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Erika Holzbaur, Andrea Stavoe

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmec-4::Chrimson::SL2::mCherry::unc-54

Plasmid

#107745

Purpose

Express Chrimson and mCherry in mechanosensory neurons.

Depositor

Andrew Leifer

Article

Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

Inserts

Chrimson
mCherry

Expression

Insect and Worm

Promoter

mec-4

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSM1959

Plasmid

#41837

Depositor

Susan Michaelis

Article

Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

Insert

SEC63-mRFP

Tags

mRFP

Expression

Yeast

Available Since

Jan. 22, 2013

Availability

Academic Institutions and Nonprofits only

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