We narrowed to 49,140 results for: Nes

1. pEN_TT 3xFLAG-MAVS-Gln93

   Plasmid

   #158629

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN_TT 3xFLAG-MAVS-Ala148

   Plasmid

   #158630

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A

   Promoter

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-SENP2 CCm

   Plasmid

   #126594

   Purpose

   Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 203-228 and siRNA resista…

   Promoter

   CMV

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCHSU74.2-2.2

   Plasmid

   #115543

   Purpose

   Expression of a fungal biosynthetic gene cluster

   Depositor

   Ronald Davis

   Article

   Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

   Insert

   S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX304 PCSK5 (R511C)-V5 blast

   Plasmid

   #83104

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Arginine 511 to Cysteine

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pLX304 PCSK5 (T288P)-V5 blast

   Plasmid

   #83101

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pLX304 PCSK5 (A270T)-V5 blast

   Plasmid

   #83102

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Alanine 270 to Threonine

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pLX304 PCSK5 (H516P)-V5 blast

   Plasmid

   #83103

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Histidine 516 to Proline

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-Hemmar-DSCP-ftz-FLP(D)

   Plasmid

   #119894

   Purpose

   FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Inserts

   FLP(D)
   DSCP-ftz (ftz Fly)
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   a mutated form of FLP, which at position 5 contai…

   Promoter

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCHKU16.1-2.2

    Plasmid

    #115529

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCHKU20.1-2.2

    Plasmid

    #115531

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCHSU68.2-2.2

    Plasmid

    #110681

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCHKU27.2-2.2

    Plasmid

    #110662

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCHSU75.2-2.2

    Plasmid

    #110691

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCHKU33.2-2.2

    Plasmid

    #110667

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX302 PCSK5 (T288P)-V5 puro

    Plasmid

    #98709

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Threonine 288 to Proline

    Promoter

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KC1G3_HUMAN_D0

    Plasmid

    #79685

    Purpose

    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G3 (CSNK1G3 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only