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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_8

Plasmid

#60568

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_8

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_11

Plasmid

#60582

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_11

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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FOR human NANOS3 TALEN

Plasmid

#59722

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG

Use

TALEN

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
REV human NANOS3 TALEN

Plasmid

#59723

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI

Use

TALEN

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pARv-RFP

Plasmid

#60021

Purpose

pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMyc4ElbLuc.

Plasmid

#53246

Purpose

reporter plasmid containing 4 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc4ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCG-Oct-1

Plasmid

#53306

Purpose

plasmid contains POU2F1 (Oct-1) gene

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pIC306

Plasmid

#52257

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska3 (SKA3 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hEWSR1WT-CMyc

Plasmid

#50733

Purpose

Produces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Lentiviral

Tags

FLAG and Myc

Promoter

TRE

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only