We narrowed to 30,074 results for: des.2

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2.aBTX

Plasmid

#122257

Purpose

Express membrane-tethered alpha-bungarotoxin in Danio rerio

Depositor

Loren Looger

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

alpha-bungarotoxin

Use

Synthetic Biology

Tags

FLAG

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAquaN1

Plasmid

#42888

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Expression

Mammalian

Mutation

T66S and H149G

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pDYU1G/γ-tubulin-mEGFP

Plasmid

#154301

Purpose

Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP

Promoter

actin15

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3

Plasmid

#115521

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3 (Senp3 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCLE NICD3

Plasmid

#26894

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD3 (NOTCH3 Human)

Use

Retroviral

Tags

Alkaline Phosphatase and IRES

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#162606

Purpose

Pre-express Cas9 in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDYU1B/mRuby3–α-tubulin

Plasmid

#154302

Purpose

Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

alpha-tubuin

Use

Dictyostelium expression

Tags

mRuby3

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

Plasmid

#102770

Purpose

"Activate neurons with 2p stimulation"

Depositor

Karel Svoboda

Article

Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

Insert

ChrimsonR::FusionRed::Kv2.1

Use

AAV

Tags

FusionRed and Kv2.1 traficking domain

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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zCD2

Plasmid

#122031

Purpose

Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Zebrafish HDAC6 Catalytic Domain 2

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-p23

Plasmid

#108225

Purpose

Mammalian expression vector for EYFP fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EYFP-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK055

Plasmid

#171327

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

rTA2S-M2 (TetR-3xVP16) transcriptional activator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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