We narrowed to 16,111 results for: grna

Showing: 9521 - 9540 of 16111 results

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPN10-gDM1d

Plasmid

#114387

Purpose

pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

Depositor

Vincent Dion

Publication

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gDM1d

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-FnCpf1

Plasmid

#129554

Purpose

Contains codon-optimized FnCpf1 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Publication

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

FnCpf1

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SaCas9v2-Puro

Plasmid

#178802

Purpose

SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB 35s:dCas:VP64:tNos (GB1189)

Plasmid

#75403

Purpose

Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

dCas:VP64

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

35S

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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PX458 eSp(1.1) V3

Plasmid

#226963

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLIII CKBs CRISPR with guides at 1-2 and 2-3

Plasmid

#238525

Purpose

Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha3

Depositor

Michael Hothorn

Insert

AtCK2alpha3 (AT2G23080 Mustard Weed)

Use

CRISPR

Expression

Plant

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLII-CRISPR for CKA4

Plasmid

#238521

Purpose

Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4

Depositor

Michael Hothorn

Insert

AtCK2alpha4 (AT2G23070 Mustard Weed)

Use

CRISPR

Expression

Plant

Available since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

Plasmid

#189943

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

Depositor

Kenneth Yamada

Publication

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

Plasmid

#184292

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

Depositor

Bernardo Sabatini

Insert

sgRNA-Grp

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

Plasmid

#184291

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

Depositor

Bernardo Sabatini

Insert

sgRNA-Oprl1

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

Plasmid

#184290

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

Depositor

Bernardo Sabatini

Insert

sgRNA-Rxfp3

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

Plasmid

#184295

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

Depositor

Bernardo Sabatini

Insert

sgRNA-Adrb1

Use

AAV and CRISPR

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170812

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Publication

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

Plasmid

#139096

Purpose

Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Publication

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dxCas9 3.7

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to scFv (anti-spike)

Plasmid

#186421

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

Depositor

Stephen Elledge

Publication

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-scFv fusion (anti-SARS-CoV-2 spike)

Use

CRISPR

Tags

6His

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Publication

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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