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pTRE:shRNA A-pGK:rtTA-Cre

Plasmid

#68471

Purpose

Inducible lentivirus expressing an arbitrary shRNA and Cre

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

GFP-shRNA A-miR30
rtTA-2A-Cre

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

pGK and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mKate2-P2A-APEX2-OSBP

Plasmid

#67664

Purpose

Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-OSBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

Plasmid

#71282

Purpose

Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Promoter

HSP70l

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCellFree_G03 PC

Plasmid

#67132

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PC (PC Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
GK Rab3A p201

Plasmid

#44696

Depositor

Geri Kreitzer

Insert

Rab3A (RAB3A Human)

Use

Gateway Cloning

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only
GFP-BRPF3

Plasmid

#65383

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF3 (BRPF3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-Cdh1

Plasmid

#49776

Purpose

pENTR vector for mouse E-cadherin

Depositor

Jamie Davies

Publication

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Cdh1 (Cdh1 Mouse)

Use

Gateway Cloning

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Publication

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSLIK Tgfbr3-HA neo

Plasmid

#58508

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Publication

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Publication

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Publication

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pSLIK MEK5-DD-mRFP1 neo

Plasmid

#47548

Purpose

Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Publication

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD-mRFP1 (MAP2K5 Human)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Mutation

S311D, T315D

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBabe_puro_DEST_Flag_SOX2

Plasmid

#45270

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
MAP2-CD4

Plasmid

#43916

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

CD4 (CD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Human MAP2 promoter

Available since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-N SV40 ST

Plasmid

#37858

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

SV40 ST (SV40gp7 Simian Virus 40)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDR-GW FLIPglu-30μΔ13V

Plasmid

#27998

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-30μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

D154A in mglB

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only