We narrowed to 75,383 results for: Ran;

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pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHC5

Plasmid

#85812

Purpose

To rescue the expression of DHHC5 in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 (XSB363)

Plasmid

#78505

Purpose

Construct for expressing Grb2 in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#210525

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-HIFR

Plasmid

#192946

Purpose

Lentiviral production of HIF-1alpha reporter

Depositor

Markus Covert

Article

Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

Insert

HIF-1alpha reporter

Use

Lentiviral

Promoter

mCMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma13-GFP2

Plasmid

#140992

Purpose

Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma13-GFP2 (GNG13 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-Flag-DHHC5

Plasmid

#85812

Purpose

To rescue the expression of DHHC5 in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PS-Grb2 (XSB363)

Plasmid

#78505

Purpose

Construct for expressing Grb2 in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Promoter

649 bp

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

Plasmid

#187588

Purpose

Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NLS

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28c(+)-YTHDF2

Plasmid

#102275

Purpose

For bacterial expression of His-tagged human YTHDF2

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF2 (YTHDF2 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 delta-F

Plasmid

#81116

Purpose

mammalian expression of F-box deleted Skp2

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deleted F box

Promoter

CMV

Available Since

Sept. 24, 2016

Availability

Academic Institutions and Nonprofits only
pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-WDR5

Plasmid

#29759

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

WDR5 (WDR5 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 29, 2013

Availability

Academic Institutions and Nonprofits only

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