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pCfB3050(gRNA XII-5)

Plasmid

#73292

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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shCDK4/6(1)

Plasmid

#73554

Purpose

vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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xfz7/pCS2+

Plasmid

#16324

Depositor

Peter Klein

Publication

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-7 (fzd7.L Frog)

Use

Vertebrate expression

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pET-AtC60αβC20NtL

Plasmid

#160876

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Publication

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1α-IRES-EGFP-hMCIDAS

Plasmid

#149696

Purpose

Expresses untagged human MCIDAS in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

MCIDAS (MCIDAS Human)

Use

Lentiviral

Promoter

EF1 alpha

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_PAX7_P2A_Hygro_Barcode

Plasmid

#120472

Purpose

Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

PAX7 (PAX7 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3050(gRNA XII-5)

Plasmid

#73292

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
shCDK4/6(1)

Plasmid

#73554

Purpose

vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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xfz7/pCS2+

Plasmid

#16324

Depositor

Peter Klein

Publication

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-7 (fzd7.L Frog)

Use

Vertebrate expression

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CDK2-3XFlag-pBabe

Plasmid

#73557

Purpose

vector encoding a fusion protein between CDK2 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK2 (CDK2 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only
pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Publication

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMB-TMEr

Plasmid

#61208

Purpose

Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry-BCP

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vinculin-23

Plasmid

#55453

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Publication

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vinculin (VCL Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pSR-puro-Exo84

Plasmid

#31116

Depositor

Christopher Counter

Publication

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Exo84 shRNA (EXOC8 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
VVVVV

Plasmid

#29426

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-5-Venus-5-Venus-5-Venus-5-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Ubp8/PET32a

Plasmid

#26965

Depositor

Cynthia Wolberger

Publication

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Ubp8 (UBP8 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

TEV site is added t the 5' end and 4 alanine…

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article