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We narrowed to 31,790 results for: son

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Showing: 9561 - 9580 of 31790 results

  1. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid
    #188901
    Purpose
    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    SID-dCas9-XTEN80-KRAB(Kox1)
    Use
    Lentiviral
    Tags
    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188901-plasmid-map-sequence-id-374829

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  2. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid
    #187276
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain
    Depositor
    Alpha Yap
    Publication
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaActin binding domain
    Promoter
    U6, CMV
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187276-plasmid-map-sequence-id-372976

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  3. canxTM-mEmerald (cytosolic)

    Plasmid
    #186962
    Purpose
    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.
    Depositor
    Ke Xu
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CANX (CANX Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Only express calnexin (482-end) and add ER signal…
    Promoter
    CMV Promoter
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    186962-plasmid-map-sequence-id-372919

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  8. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

    Plasmid
    #154303
    Purpose
    Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum
    Depositor
    Shigehiko Yumura
    Publication
    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    histone H1
    γ-tubulin
    Use
    Dictyostelium expression
    Tags
    mEGFP and mRuby3
    Promoter
    actin15
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    154303-plasmid-map-sequence-id-336453

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  12. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid
    #140202
    Purpose
    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140202-plasmid-map-sequence-id-310118

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  14. LSB-hsa-miR-29a-3p

    Plasmid
    #103391
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Publication
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-29a-3p target (MIR29A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    103391-plasmid-map-sequence-id-195831

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  16. pcDNA3 RL3Sc

    Plasmid
    #109365
    Purpose
    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    Tags
    1D4
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109365-plasmid-map-sequence-id-210146

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  18. CIAR_pcDNA5/FRT/TO

    Plasmid
    #86502
    Purpose
    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.
    Depositor
    Dustin Maly
    Publication
    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIAR (SOS1 Human)
    Use
    Frt
    Expression
    Mammalian
    Mutation
    T968L in SOScat
    Promoter
    CMV (tet operator)
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86502-plasmid-map-sequence-id-171532

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  19. pGEX6P1-human LIC1 C-terminal half

    Plasmid
    #74599
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
    Depositor
    Ron Vale
    Publication
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    T7
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    74599-plasmid-map-sequence-id-145961

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  20. mEos4b-LaminA-C-18

    Plasmid
    #57511
    Purpose
    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LaminA (LMNA Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9561 - 9580 of 31790 results