We narrowed to 8,089 results for: KRAS;
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Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
TagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 742 (pDONR221-EGFP-KRAS4B-G12V)
Plasmid#156403PurposeGateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseGateway cloningTagsEGFPMutationmutant KRAS4B-G12VAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD
Plasmid#196086PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.DepositorInsertgRNA targeting lncRNA NORAD
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156410PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12VDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
TagsmScarlet-IExpressionMammalianMutationmutant KRAS4A-G12VPromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
TagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPPromoterEF1aAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196079PurposeConstitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196085PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1
Plasmid#176537PurposeNon-protein coding locus components from KraIscB-1DepositorInsertnon-coding locus components from KraIscB-1 locus
ExpressionBacterialAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196076Purpose(Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
ExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196080Purpose(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Blasticidin S deaminase
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196074Purpose(Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
ExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
R713-M48-303: CMV51p> Halotag-Kras4aHVR
Plasmid#159684PurposeMammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusionDepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R703-M80-303: CMV51p> Halotag-Kras4bHVR
Plasmid#159683PurposeMammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusionDepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: Venus-oLID-CAAX (from KRas4B)
Plasmid#60412PurposeMammalian Expression of Venus-oLID-CAAXDepositorInsertVenus-oLID-CAAX
UseCre/Lox and LentiviralTagsVenusExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156406PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseGateway cloningTagsmScarlet-IMutationmutant KRAS4A-G12VAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPMutationmutant KRAS4B-G12VPromoterkdrlAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only