Addgene

We narrowed to 4,495 results for: PGK

Showing: 941 - 960 of 4495 results

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MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Expression

Mutation

Human codon-optimized

Promoter

mouse PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE

Plasmid

#46601

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-223 Decoy (MIR223, Mir223 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

Plasmid

#46617

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-375 Decoy (MIR375, Mir375 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

Plasmid

#46590

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-146a Decoy (MIR146A, Mir146 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pCWX-R4DESTR2-PGK-PURO

Plasmid

#114319

Purpose

Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Puromycin resistance

Depositor

Patrick Salmon

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

Plasmid

#105019

Purpose

Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps39 (Vps39 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

Plasmid

#159287

Purpose

Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT7 (ID. 5) (KAT7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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ACTIN-Cxcl5-PGK-Cre

Plasmid

#110278

Purpose

Bicistronic lentivirus expressing Cxcl5 and Cre

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Inserts

chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
cyclization recombinase

Use

Cre/Lox and Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

B-actin and Pgk

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-145-Decoy.hPGK.GFP.WPRE

Plasmid

#46589

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-145 Decoy (MIR145, Mir145a Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-210-Decoy.hPGK.GFP.WPRE

Plasmid

#46599

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-210 Decoy (MIR210, Mir210 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-FLAG-TAT-86-E

Plasmid

#35978

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "E" HIV clade

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.U6.miR-325-Decoy.hPGK.GFP.WPRE

Plasmid

#46610

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-325 Decoy

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-27b-Decoy.hPGK.GFP.WPRE

Plasmid

#46603

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-27b Decoy (MIR27B, Mir27b Human, Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-125b-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46582

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-125b-3p Decoy

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only