We narrowed to 4,065 results for: SPL;
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Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianPromoterSynapsinAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-MLPmin-luc2
Plasmid#194383PurposeReporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoterDepositorInsert10xUAS-luc2
ExpressionMammalianPromoterMLP minimalAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]
Plasmid#133324PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of NTD in full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-FRB-IRES-mCherry
Plasmid#136602PurposeEncodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-FRB
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPa-FRB-pre mGRASP
Plasmid#73145PurposeLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.DepositorInsertsHRPa-FRB-pre mGRASP
TagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry
Plasmid#136603PurposeEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertFKBP-C-mSOG-J-alpha
ExpressionMammalianPromoterCMVAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cTRb413
Plasmid#188261PurposePlasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST
Plasmid#194386PurposeGateway Destination vector for GPCR split TEV assays with V2R tailDepositorInsertattR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
Tags2xHAExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBS1084 GFP-TDP43 deltaCTD
Plasmid#118798PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
P9_TEVs_cLuc
Plasmid#119303PurposeExpresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc connected to P9 coil via TEVs cleavable linker
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bJun-C-mSOG-IRES-mCherry
Plasmid#136600PurposeEncodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertbJun-C-mSOG
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9751-BEAR-mCherry-preedited
Plasmid#162993PurposeBEAR control plasmid with split mCherry and intact 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]
Plasmid#133329PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll FYW to S in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-bFos-IRES-mCherry
Plasmid#136599PurposeEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-bFos
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS1413 GFP-TDP43 VLIM-F
Plasmid#118802PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll VLIM to F in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta-delta10
Plasmid#52187PurposeExpresses human ERRbeta-delta10 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS1411 GFP-TDP43 FYW-S
Plasmid#118800PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to S in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1415 GFP-TDP43 VLIM-S
Plasmid#133323PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only