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pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#226475

Purpose

Improved microglia-targeted transgene expression

Depositor

Hirokazu Hirai

Article

Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
target sequences for miR-9x4 & miR-129-2-3px4 *2
EGFP

Use

AAV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC cloning vector

Plasmid

#10878

Purpose

3rd generation transfer plasmid

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC control

Plasmid

#10879

Purpose

Negative control shRNA vector containing non-hairpin insert.

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Insert

non-hairpin 18bp

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB402-omega

Plasmid

#74797

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_GPHN.FingR-mKate2-IL2RGTC

Plasmid

#46297

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_GPHN.FingR-eGFP-CCR5TC

Plasmid

#46296

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Has Service

AAV1

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

Plasmid

#184635

Purpose

Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Klf2-t2A-mCherry

Plasmid

#127539

Purpose

Expresses mouse Klf2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Klf2 (Klf2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Expression

Mammalian

Mutation

R203M

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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CoV2-N-WT-Hu1

Plasmid

#177937

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB502-omega

Plasmid

#74845

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomArchon

Plasmid

#126943

Purpose

Cre-dependent expression of SomArchon under CAG promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD_His B_QuasAr2

Plasmid

#64134

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2

Expression

Bacterial

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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