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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,424 results for: dcas9

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Showing: 941 - 960 of 2424 results

  2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  3. pAPH07

    Plasmid
    #234345
    Purpose
    Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK
    Depositor
    Jerome Fox
    Publication
    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12
    Insert
    Type II dCas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lacUV5
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234345-plasmid-map-sequence-id-470060

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  4. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid
    #209298
    Purpose
    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
    Depositor
    Birgitt Schuele
    Publication
    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1
    Inserts
    dCas9
    tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    tetracycline-inducible promoter (TRE3G)
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    209298-plasmid-map-sequence-id-414164

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  9. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Publication
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  10. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Publication
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    159086-plasmid-map-sequence-id-310691

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Showing: 941 - 960 of 2424 results