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GFP-PKCBII

Plasmid

#195232

Purpose

Mammalian expression vector containing GFP tagged PKCBII.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB2 (PRKCB Human)

Expression

Mammalian

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-LMNB1-IRES2-mCherry

Plasmid

#159601

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-LMNB1-IRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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459 p3E-2A-nlsEGFPpA

Plasmid

#195969

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsEGFP (nuclear)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCK051 p5E exorh:mCerulean

Plasmid

#195952

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB HA cIAP1 H588A

Plasmid

#38233

Depositor

Colin Duckett

Article

Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

Insert

cIAP1 H588A (BIRC2 Human)

Tags

HA

Expression

Mammalian

Mutation

H588A

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pDOC-K-glmS-GFPfor

Plasmid

#158059

Purpose

Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

Green Fluorescent Protein optimised for excitation with UV light

Use

Synthetic Biology

Expression

Bacterial

Promoter

acpP

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCK052 p5E exorh:mCherry

Plasmid

#195953

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCherry

Use

Other

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Article

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Tags

His tag

Expression

Bacterial

Promoter

lac operon

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-K45Q-GFP

Plasmid

#208777

Purpose

MSCV vector expressing K45Q mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Change Lys 45 to Gln (K45Q)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

Plasmid

#159927

Purpose

Gene targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Insert

ERT2CreERT2

Use

Marmoset targeting

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCK081 desmaMCS:minpromCerulean,exorh:mCherry

Plasmid

#200014

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available Since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Consensus

Plasmid

#176664

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-PKCB1 K371R

Plasmid

#195230

Purpose

Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB1 (PRKCB Human)

Use

Retroviral

Mutation

K371R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Changed aspartic acid 53 to alanine (D53A)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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