We narrowed to 3,278 results for: multiplex

1. pRoLuc

   Plasmid

   #135284

   Purpose

   GBPart - RoLuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   R. ohbai Luciferase
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_D10A-1x5

   Plasmid

   #58775

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-luxKOk

   Plasmid

   #229944

   Purpose

   Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKOk
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-luxGold

   Plasmid

   #229943

   Purpose

   Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxGold
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAWp28

   Plasmid

   #73850

   Purpose

   pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Type

   Empty backbone

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Plasmid

   #163073

   Purpose

   Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Grx1
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCasTns(Ptr)-IS186

   Plasmid

   #175581

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
   

   Expression

   Bacterial

   Mutation

   NO

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

   Plasmid

   #182009

   Purpose

   CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   IgKchL_HA_STf_myc_PDGFRtmb
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCl-MCS_miniCMV_miRFP703

   Plasmid

   #134985

   Purpose

   The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   miRFP703
   

   Use

   Lentiviral

   Promoter

   minCMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p32Fn

    Plasmid

    #158153

    Purpose

    Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Rice codon optimized

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTubulin-miRFP670nano

    Plasmid

    #127429

    Purpose

    Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-human alpha-tubulin
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgNeo2/Cre

    Plasmid

    #89652

    Purpose

    Expresses a Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Neomycin
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWS5398-CRISPRai-HygR

    Plasmid

    #182837

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWS5390-CRISPRai-URA3

    Plasmid

    #182829

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ265_OoA3GX2

    Plasmid

    #225147

    Purpose

    Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    OoA3GX2-zCas9(D10A)-UGI
    

    Expression

    Plant

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCl-MCS_miniCMV_TagBFP

    Plasmid

    #134986

    Purpose

    The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgNT3/Cre

    Plasmid

    #89654

    Purpose

    Expresses a non-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    non-targeting gRNA
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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