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Showing: 941 - 960 of 13832 results

  3. GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid
    #166986
    Purpose
    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(1), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    166986-plasmid-map-sequence-id-330477

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  4. GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166991
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    166991-plasmid-map-sequence-id-330472

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  5. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid
    #166990
    Purpose
    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(1), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    166990-plasmid-map-sequence-id-330470

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  6. GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166987
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166987-plasmid-map-sequence-id-329691

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  8. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid
    #160887
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    PT7-Nt-rbcS-T4a
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T4a: T7, chaperones: T10
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160887-plasmid-map-sequence-id-318851

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  13. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69024-plasmid-map-sequence-id-127982

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  14. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  17. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  18. pAL113

    Plasmid
    #22471
    Purpose
    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB NT (PHYB Mouse, Mustard Weed)
    Use
    Synthetic Biology
    Tags
    10aaLinker-mCFP-HrasCT
    Expression
    Mammalian
    Mutation
    Y276H
    Promoter
    CMV
    Available since
    Nov. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    22471-plasmid-map-sequence-id-10325

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Showing: 941 - 960 of 13832 results