166,818 results

1. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp6-mCherry

   Plasmid

   #165133

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp6 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY094-EFS-CD22-Blast-WPRE

   Plasmid

   #192195

   Purpose

   Lenti-CD22-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-CD22-OE-Blast (CD22 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IB-TMEM192-mKeima

   Plasmid

   #215359

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NUMB2-eGFP-M107K

   Plasmid

   #37803

   Depositor

   Rolf Bjerkvig

   Article

   Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

   Insert

   NUMB isoform 2 (NUMB Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   pCMV

   Available Since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121184

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   CMV-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Potential silent point mutation in GFP sequence

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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