We narrowed to 23,031 results for: his

Showing: 9601 - 9620 of 23031 results

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pRBC-sfGFP-134/150TAG

Plasmid

#174077

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Mutation

D134TAG + N150TAG

Promoter

T7

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx

Plasmid

#36944

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-dCLK

Plasmid

#33153

Depositor

Michael Rosbash

Article

McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

Insert

CLK (Clk Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
PB_TRE_sg61-PDGFR_EF1a_eGFP

Plasmid

#186310

Purpose

Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg61-PDGFR
eGFP

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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Human pIgR(SC)

Plasmid

#158216

Purpose

Express the ectdomain (SC) of human pIgR

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

PIGR (PIGR Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBADmod1-linker2-gamS

Plasmid

#45833

Depositor

Richard Murray, Vincent Noireaux

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

gamS

Use

Synthetic Biology

Tags

Histag

Expression

Bacterial

Promoter

pBAD

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Article

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Tags

His tag

Expression

Bacterial

Promoter

TRC

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pET28a T7pCONS TIR-1 sfGFP

Plasmid

#154463

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-Arl13b11

Plasmid

#40873

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Tags

His and V5

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CYP2D6

Plasmid

#67090

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2D6 (CYP2D6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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