We narrowed to 11,122 results for: HA

1. psiCHECK-1-miR-376a1(3p)-I-type

   Plasmid

   #215883

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK-1-miR-589(5p)-I-type

   Plasmid

   #215884

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK-1-miR-589(3p)-I-type

   Plasmid

   #215885

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCHECK-1-miR-22(3p)-I-type

   Plasmid

   #215886

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-1-miR-376a2(3p)-I-type

   Plasmid

   #215887

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-1-miR-99a-I-type

   Plasmid

   #215888

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_SRGAP2A_SH3(W765A)_HA

   Plasmid

   #222614

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   Synapsine promoter and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   CMV and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FKBP-ARHGAP45

    Plasmid

    #221301

    Purpose

    Expression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    ARHGAP45 (pLxIS) (Hmha1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG-4xFKBP

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCHECK-VIM270-pCM

    Plasmid

    #215869

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. B2M-SDMutation-EhT

    Plasmid

    #215546

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    

    Use

    CRISPR

    Mutation

    The second base of the first intron of B2M (From …

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. B2M-SDMutation-EPG

    Plasmid

    #215545

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    

    Use

    CRISPR

    Mutation

    The second base of the first intron of B2M (From …

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-SRRD-noTag (in pLEX_307)

    Plasmid

    #214919

    Purpose

    constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    SRRD (SRRD Human)
    

    Use

    Lentiviral

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL4-Creb5 (1- 128) T59/T61 A

    Plasmid

    #195032

    Purpose

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
    

    Expression

    Mammalian

    Mutation

    T59/T61 A

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Tags

    HA signal FALG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pet28b_His_RAD23A-UBA1

    Plasmid

    #201549

    Purpose

    Expresses UBA1 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 160-205 of DNA polymerase i…

    Promoter

    T7

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_PGK_3xHA-LTB4R(T308A,S310A)

    Plasmid

    #208094

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R(T308A,S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T308A, S310A

    Promoter

    PGK

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-EphB4

    Plasmid

    #200983

    Purpose

    Mammalian expression plasmid for myc-tagged EphB4

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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