We narrowed to 12,185 results for: SHA

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTXBI-mClover-YTH domain

Plasmid

#177123

Purpose

Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTH domain) (YTHDC1 Human)

Tags

Mxe GyrA intein-Chitin binding domain for protein…

Expression

Bacterial

Mutation

YTH domain only (IDR1 and IDR2 deletion)

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-C1V1-eYFP

Plasmid

#135633

Purpose

AAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, AAV5, and AAV9

Insert

C1V1-eYFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

Plasmid

#184556

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-E29-ChR2GFP2x

Plasmid

#153437

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-PR.Cre

Plasmid

#192933

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-mEGFP-PHOX2B-WT

Plasmid

#194576

Purpose

Mammalian Expression of mEGFP-PHOX2B WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry-Cbx5

Plasmid

#194547

Purpose

Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

Cbx9 (HP1a)

Tags

mCherry

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pABVec-NbH11-D4_huIgG1

Plasmid

#160314

Purpose

Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH D4-Fc

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-mEGFP-PHOX2B-MUT

Plasmid

#194577

Purpose

Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

S207Afs*102

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGGG-Sr43

Plasmid

#186974

Purpose

Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

Depositor

Brande Wulff

Article

Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

Insert

Sr43

Tags

None

Expression

Bacterial and Plant

Mutation

None

Promoter

Natice

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RAX-WT

Plasmid

#194572

Purpose

Mammalian Expression of mEGFP-RAX WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E5-dTom-nlsdTom

Plasmid

#135640

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E5 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Tomato MEF2C

Plasmid

#89715

Purpose

Retroviral gene expression vector for MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-E22-ChR2GFP2x

Plasmid

#153436

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E22 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pABVec-NbH11-H4_huIgG1

Plasmid

#160313

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH H4-Fc

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

Plasmid

#114427

Purpose

Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tTA2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

PGK, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai133 (TITL-ssAPEX2tm) targeting vector

Plasmid

#114432

Purpose

Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ssAPEX2tm

Use

Cre/Lox and Mouse Targeting

Promoter

TREtight

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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