We narrowed to 23,664 results for: CRISPR

1. pBluescriptSKII+ U6-sgRNA(F+E) empty

   Plasmid

   #74707

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
   

   Expression

   Mammalian

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK-caCas9-NatMX-Neut5L

   Plasmid

   #89576

   Purpose

   caCas9 integrating vector into the Neut5L locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pST1374-NLS-flag-linker-Cas9

   Plasmid

   #44758

   Depositor

   Xingxu Huang

   Article

   Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

   Insert

   Human codon optimized Cas9
   

   Use

   CRISPR

   Tags

   32 amino acid linker, Flag, and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_KLF9

   Plasmid

   #86286

   Purpose

   Donor vector for 3' FLAG tag of human KLF9

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   KLF9 homology arms (KLF9 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

   Plasmid

   #149722

   Purpose

   Lentiviral expression of crRNA with GFP and a neomycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mH6-Cas12c1

   Plasmid

   #120872

   Purpose

   Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. G1333 DddAtox-C–dSpCas9

   Plasmid

   #157834

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mH6-Cas12i2

    Plasmid

    #120883

    Purpose

    Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV/eGFP-U6-sgRNA

    Plasmid

    #170544

    Purpose

    A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132410

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dEsCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R295A; H300A; R849A; H854A

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

    Plasmid

    #121448

    Purpose

    To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

    Depositor

    Brian Chen

    Article

    Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

    Insert

    RFPnols-PQR-ZeocinR (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SiC-V2-Cas9G7

    Plasmid

    #133043

    Purpose

    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    Tet repressor
    

    Use

    Lentiviral

    Tags

    Cerulean

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFETCh_AHR

    Plasmid

    #101071

    Purpose

    Donor vector for 3' FLAG tag of human AHR

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    AHR homology arms (AHR Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

    Plasmid

    #132403

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG2_hph

    Plasmid

    #184915

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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