We narrowed to 24,091 results for: CRISPR
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Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180266PurposeLentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNADepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KLF9
Plasmid#86286PurposeDonor vector for 3' FLAG tag of human KLF9DepositorAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044AAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
ExpressionMammalianPromoterU6Available SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectPromoterAe. aegypti PUb and endogenous AGO1Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H3C2
Plasmid#207780PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.DepositorAvailable SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128346PurposepAAV encoding control gRNA for CRISPR gRNAs listed aboveDepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only