We narrowed to 16,015 results for: grn

1. YN2_1_LT58_X2site

   Plasmid

   #177705

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. bRA89

   Plasmid

   #100950

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-J23109

   Plasmid

   #113151

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWS5399-CRISPRai-ZeoR

   Plasmid

   #182838

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p201B Cas9

   Plasmid

   #59177

   Purpose

   Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   BAR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. ttAtCas12a+int

   Plasmid

   #182384

   Purpose

   Gives very high editing efficiency in barley. Also works in Brassica oleracea

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   LbCas12a coding sequence with D156R and Arabidopsis introns
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R + 8 Arabidopsis introns added

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRN1120

   Plasmid

   #101750

   Purpose

   Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ134B2.0

   Plasmid

   #167158

   Purpose

   Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GEARBOCS-SPARCL1-N-mCherryTag

    Plasmid

    #218186

    Purpose

    To tag Hevin with mCherry at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWS5321-CRISPRai-Markerless

    Plasmid

    #182828

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - markerless

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS143

    Plasmid

    #122377

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. bRA90

    Plasmid

    #100951

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge