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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,432 results for: tes

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Showing: 9681 - 9700 of 17432 results

  3. mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

    Plasmid
    #220075
    Purpose
    expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP
    Depositor
    Gerry Hammond
    Publication
    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OSBPL5 H478/9A (OSBPL5 Human)
    Expression
    Mammalian
    Mutation
    H478/9A, deltaTMD
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220075-plasmid-map-sequence-id-434122

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  4. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  7. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

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  9. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  11. SNAP-CLC-EGFP

    Plasmid
    #193579
    Purpose
    Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.
    Depositor
    Justin Taraska
    Publication
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clathrin light chain B (CLTB Human)
    Tags
    SNAP-tag and mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193579-plasmid-map-sequence-id-380177

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  13. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

    Plasmid
    #178868
    Purpose
    Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA
    Depositor
    Shinya Tsukiji
    Publication
    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178868-plasmid-map-sequence-id-355800

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  14. pIRESneo2 mycBioID-Tollip

    Plasmid
    #178054
    Purpose
    myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Publication
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    mycBioID
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178054-plasmid-map-sequence-id-350117

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  16. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

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  18. pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

    Plasmid
    #155195
    Purpose
    Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus
    Depositor
    Kevin Park
    Publication
    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d
    Insert
    Thrombospondin-1 (Thbs1 Mouse)
    Use
    AAV
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    THBS1 with deletion of amino acid residues 276-315
    Promoter
    CMV
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    155195-plasmid-map-sequence-id-309128

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Showing: 9681 - 9700 of 17432 results