We narrowed to 23,671 results for: CRISPR

1. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

   Plasmid

   #91918

   Purpose

   Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbfCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ220 (AsCpf1)

   Plasmid

   #86208

   Purpose

   AsCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   AsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   5' and 3' NLS

   Expression

   Plant

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132408

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLbaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R600A; H605A; R1243A; H1248A

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Promoter

   Chicken ß-actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV_AA089

   Plasmid

   #216105

   Purpose

   gRNA expression for Cas12a (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

   Plasmid

   #222992

   Purpose

   Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180268

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   GARP sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

   Plasmid

   #166873

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA array presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAsCpf1(TYCV)(BB) (pY211)

   Plasmid

   #89352

   Purpose

   Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CBh

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_KDM1A

    Plasmid

    #86260

    Purpose

    Donor vector for 3' FLAG tag of human KDM1A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KDM1A homology arms (KDM1A Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV537

    Plasmid

    #177704

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX552-EF1a-DIO-mcherry(with gRNA scaffold)

    Plasmid

    #199581

    Purpose

    Expresses mCherry in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    mCherry
    

    Use

    AAV, CRISPR, and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a intron A and U6

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

    Plasmid

    #168153

    Purpose

    Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ230-E795L

    Plasmid

    #176890

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    LbCas12a-E795L
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. gh90

    Plasmid

    #106761

    Purpose

    expression of gRNA targeting FUT6

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT6 (FUT6 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

    Plasmid

    #129530

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132412

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dAdmCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R269A; H274A; R837A; H842A

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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