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pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.Tau2N4R

Plasmid

#226396

Purpose

Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP:FRB-FBAR

Plasmid

#223620

Purpose

Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

FBP17 (FNBP1 Human)

Tags

FKBP, TagBFP and mGFP, FRB

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.8D2Q.Tau2N4R

Plasmid

#226398

Purpose

Expresses his-tagged, 3C-cleavable 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-long

Plasmid

#210642

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Lentiviral

Tags

GFP

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-short

Plasmid

#210641

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Lentiviral

Tags

GFP

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG_HA-Regnase-1 D141N

Plasmid

#222663

Purpose

Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

SFFV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-EGFP-NPM1

Plasmid

#238268

Purpose

For overexpression of VHH05-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Article

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

Plasmid

#233506

Purpose

Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

DsRed loxp eGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238236

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-p65

Plasmid

#111190

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-GFP-nb-KS

Plasmid

#238260

Purpose

For overexpression of mCherry-GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-puro-PTPIP51(1-150_175-470)

Plasmid

#170537

Purpose

Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Mutation

deleted amino acids 151-175

Promoter

CAG promoter

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only
pLEVI(408)-ColE-iLight-msfGFP

Plasmid

#170268

Purpose

Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Tags

msfGFP

Expression

Bacterial

Mutation

To design iLight for expression in bacteria, a fu…

Promoter

pJ23116

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

Plasmid

#162278

Purpose

Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN1A Variant 1, stabilized (SCN1A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp2946. Silent codon c…

Promoter

CMV

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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