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LR (bact: HyPer)

Plasmid

#162069

Purpose

Used for the diffuse visualization of H2O2 production in cells.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

beta actin 2 (actb2 Zebrafish)

Use

Gateway Cloning

Tags

HyPer

Promoter

T3

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18UBD

Plasmid

#119005

Purpose

Mammalian expression of the human Rad18 Ubiquitin binding domain and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEE389

Plasmid

#149287

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pEE495

Plasmid

#149291

Purpose

T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEE387

Plasmid

#149285

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-hSOX2-EGFP-PuroTK

Plasmid

#141026

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1394

Plasmid

#129522

Purpose

pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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S13_EcoRV_UBC_AscI

Plasmid

#128477

Purpose

UBC promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

UBC promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAGH23

Plasmid

#107262

Purpose

contains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

curT (slr0483 )

Use

Synthetic Biology

Tags

mTurquoise2

Expression

Bacterial

Promoter

native CurT promoter

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway Cloning

Promoter

H1, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NG

Plasmid

#90423

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NG

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NN

Plasmid

#90421

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NN

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTlpAreverse-Wasabi

Plasmid

#86114

Purpose

mWasabi reporter gene driven by TlpA promoter in reverse orientation

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA (in reverse orientation)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE4

Plasmid

#79443

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE62

Plasmid

#79444

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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