We narrowed to 11,626 results for: VARS

1. pYC34

   Plasmid

   #63915

   Purpose

   Recipient overexpressing vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   TEF1 (Saccharomyces cerevisiae)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hRedOpsin-TGFB2

   Plasmid

   #164428

   Purpose

   AAV plasmid expressing TGFB2 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

   Insert

   Tgfb2 (Tgfb2 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human red opsin

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pES134

   Plasmid

   #127551

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Gavin Thomas, Emmanuele Severi

   Article

   Wu et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   F3-flanked spectinomycin resistance cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIAA5::LUC

   Plasmid

   #141169

   Purpose

   Luminescent reporter for auxin signaling

   Depositor

   Patrick Schäfer, Vardis Ntoukakis

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtIAA5 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtIAA5, At1g15580

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-GFP(+24)

   Plasmid

   #199163

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+24)
   

   Tags

   MGHHHHHHGGA

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB036d

    Plasmid

    #99216

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and PproA

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. P303-FBP_2

    Plasmid

    #139693

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB036a

    Plasmid

    #99215

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK and PproD

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-ND4 Right-GSVG-UGI

    Plasmid

    #187414

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-ND4 Right-E1347A-UGI

    Plasmid

    #187415

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. wt HA-dynamin 1 pUHD10-3

    Plasmid

    #34688

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1L Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

17. UAS::PTPRFa.1-DN-GFP

    Plasmid

    #37110

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
    

    Use

    Tol2 gateway expression vector

    Tags

    EGFP

    Mutation

    deleted amino acids 1326-1894

    Promoter

    UAS-E1b

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH760-CEN-mCherry_v3

    Plasmid

    #40598

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSC201

    Plasmid

    #129387

    Purpose

    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

    Depositor

    Silvia Cardona

    Article

    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

    Insert

    oriR6K and oriT
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    N/A

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYC121

    Plasmid

    #63917

    Purpose

    Recipient overexpressing vector to construct MYC tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    MYC

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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