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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AA184

Plasmid

#216047

Purpose

Fragmid fragment: (C' terminus) for prime editing; using PEmax

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA063

Plasmid

#216020

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-SpRY (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA061

Plasmid

#216018

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-NG (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA062

Plasmid

#216019

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9-SpG (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA060

Plasmid

#216017

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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HMGA2 shRNA

Plasmid

#25408

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-H63S

Plasmid

#82035

Purpose

Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Mutation

H63S MRE11 mutant

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

Plasmid

#138983

Purpose

IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

Plasmid

#138987

Purpose

IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

Plasmid

#138985

Purpose

IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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