We narrowed to 13,850 results for: sequence
-
Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128345PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160-QES.04
Plasmid#100933PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q842.d12) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations P124D;F…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160-QES.i04.I181L
Plasmid#111837PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q842.d12) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations P124D;I…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q769gp160-QES.i03.V255M
Plasmid#111838PurposeMammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q769.d22) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations A200E;F…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-ShhN-Cys
Plasmid#121136PurposeExpresses ShhN with a C-terminal cysteine residues in bacteriaDepositorInsertshh (Shh Mouse)
TagsHIS, HRV3C site, and MBPExpressionBacterialMutationAmino acid sequence from 26-191, with an addition…PromotertacAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 d835
Plasmid#113959Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-834PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.a-AP-His
Plasmid#72054PurposeExpresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoM-OG241
Plasmid#72842PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.DepositorInsertPatoM
ExpressionBacterialPromoterPatoMAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP7.v2 puro
Plasmid#87795PurposeExpresses an inducible short hairpin targeting human MKP7 sequenceDepositorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)
Plasmid#83463PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)DepositorAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)
Plasmid#70195PurposePost cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vectorDepositorInserttetR-deGFP fusion
Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sdk2-Fc-His
Plasmid#72135PurposeExpresses the extracellular region of the Sdk2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(S)-Fc-His
Plasmid#72152PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5a.a-AP-His
Plasmid#72050PurposeExpresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only