Addgene

We narrowed to 32,562 results for: IND

Showing: 961 - 980 of 32562 results

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pML33

Plasmid

#206996

Purpose

Contains OsTiR1 ORF and LoxP and Lox2272 sites.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

OsTiR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML58

Plasmid

#206997

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and hygromycin b resistance gene.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

HygR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML60

Plasmid

#206998

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and puromycin resistance gene.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

PuroR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML646

Plasmid

#206999

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and blasticidin S resistance gene.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

BSD resistance

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

Plasmid

#204724

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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ANXA11_Halo_C_allele

Plasmid

#178136

Purpose

Donor vector for endogenous tagging of human ANXA11 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ANXA11 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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FXN_Halo_N_allele

Plasmid

#178141

Purpose

Donor vector for endogenous tagging of human FXN at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FXN Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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GRN_Halo_C_allele

Plasmid

#178125

Purpose

Donor vector for endogenous tagging of human GRN at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (GRN Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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RAB_Halo_C_allele

Plasmid

#178124

Purpose

Donor vector for endogenous tagging of human RAB at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (AGFG1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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COQ2_Halo_N_allele

Plasmid

#178154

Purpose

Donor vector for endogenous tagging of human COQ2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (COQ2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC.B2.MPN330

Plasmid

#11536

Depositor

Sung-Hou Kim

Article

Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

Insert

FAD synthetase

Use

Tags

HisTag, non-cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

Plasmid

#58437

Purpose

Expresses LexA-ER-B112 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B112

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Puro

Plasmid

#85966

Purpose

Tet/Dox inducible shRNA lentivirus with puromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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PB-TO-MYOD1-shOct4

Plasmid

#182309

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

MYOD1-shOct4 (MYOD1 Human)
puro-T2A-mycNLS-mTagBFP2

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

shOct4

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

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pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTK21

Plasmid

#37396

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Ran (RAN Human)

Use

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

T24N

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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