Addgene

We narrowed to 2,287 results for: SRS;

Showing: 961 - 980 of 2287 results

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TRE-MPRA_minCMV Library

Pooled Library

#201012

Purpose

Massively parallel reporter assay (MPRA) library with minCMV promoter

Depositor

Justin English

Article

Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

Expression

Mammalian

Use

Luciferase

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only
TRE-MPRA_minTK Library

Pooled Library

#201014

Purpose

Massively parallel reporter assay (MPRA) library with minTK promoter

Depositor

Justin English

Article

Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

Expression

Mammalian

Use

Luciferase

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only
TRE-MPRA_minPromega Library

Pooled Library

#201013

Purpose

Massively parallel reporter assay (MPRA) library with minPromega promoter

Depositor

Justin English

Article

Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

Expression

Mammalian

Use

Luciferase

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only
pDONR223_LacZ

Plasmid

#25893

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

LacZ

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 1 tet pLKO puro

Plasmid

#162985

Purpose

Tet-inducible shRNA targeting human METTL3 #1

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti_EFS-Cas9-P2A-HygR

Plasmid

#164134

Purpose

Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2-mNeonGreen

Plasmid

#228865

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA and mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Expression

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124428

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

GENIE Project, Douglas Kim

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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