-
-
-
-
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPelt-2Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
KA2880_pPB-TREtight-FLAG-Esrrb-InO
Plasmid#124178PurposepiggyBac-based Dox-inducible expression of FLAG-EsrrbDepositorInsertFLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2944_pPB-TREtight-Esrrb-FLAG-InO
Plasmid#124179PurposepiggyBac-based Dox-inducible expression of Esrrb-FLAGDepositorInsertEsrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-rS2d-HexaPro
Plasmid#183515PurposeMammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987PDepositorInsertSpike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PT2/C-Luc//PGK-SB13
Plasmid#20207DepositorInsertFlirefly luciferase/Sb transposase
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
PT2/C-fluc
Plasmid#20203DepositorInsertC-Fluc
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT/CMV-SV40-LgT
Plasmid#20204DepositorInsertSV40LgT
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
SMA-Cherry
Plasmid#110209PurposeMapping of mesenchymal progenitor cells.DepositorInsertmCherry
UseTagsNoneExpressionMammalianMutationPromoterSmooth muscle alpha-actin and first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22 Lenti_2
Plasmid#209556PurposepU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRBDepositorInsertSpCas9 sgRNA, TadA-8e (2-76)-2xFRB
UseTagsExpressionMammalianMutationPromoterpU6, pCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-5
Plasmid#220442PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)DepositorInsertPUF3 (puf-3 Nematode)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ2
Plasmid#198173PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 μ2
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF14 C417A_IRES-mCherry
Plasmid#198385PurposeRNF14 C417A expressionDepositorInsertRNF14 (RNF14 Human)
UseLentiviralTagsV5ExpressionMammalianMutationcatalytic cysteine mutation (C417A)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF25 dRING_IRES-mCherry
Plasmid#198387PurposeRNF25 (?RING) expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationRING deletion (135-201)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
UseTagsHA and tagBFPExpressionMammalianMutationPromoterSV40Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
1105-Desmoglein2-FLAG-eGFP
Plasmid#36989DepositorInsertDsg2 (DSG2 Human)
UseTagsFLAG and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only
