We narrowed to 1,687 results for: plasmid for e coli

1. pEC2861 (pSpy1C-PgyrA_ffluc)

   Plasmid

   #218511

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PgyrA

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-TurboID-3XNLS

   Plasmid

   #209392

   Purpose

   Transiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-3XNLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGG-GFP-PM-TurboID

   Plasmid

   #209402

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   cTP-3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG-PM-TurboID

   Plasmid

   #209395

   Purpose

   Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   PM-3XHA-TurbOID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGG-cTP-TurboID

   Plasmid

   #209394

   Purpose

   Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   cTP-3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG-NES-TurboID

   Plasmid

   #209393

   Purpose

   Transiently expressing TurboID fused with a nuclear export signal (NES) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   NES-3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG-GTS-TurboID

   Plasmid

   #209397

   Purpose

   Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   GTS-3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC011

   Plasmid

   #171143

   Purpose

   Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MP4

   Plasmid

   #69652

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-HIS-Sangamo

    Plasmid

    #40786

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    N Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MP2

    Plasmid

    #69628

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MP3

    Plasmid

    #69650

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE-SUMO CRYBB2

    Plasmid

    #80749

    Purpose

    Expression of human beta B2-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYBB2 (CRYBB2 Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pE-SUMO CRYBB3

    Plasmid

    #80750

    Purpose

    Expression of human beta B3-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYBB3 (CRYBB3 Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid

    #239198

    Purpose

    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

    Depositor

    Michael O'Donnell

    Insert

    Pol3 (POL3 Budding Yeast)
    

    Use

    Tags

    3X FLAG

    Expression

    Yeast

    Mutation

    E. coli codon bias (synthetic gene), but same ami…

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T_CIP2A_1-560_R522D

    Plasmid

    #217923

    Purpose

    E. coli expression of GST-tagged CIP2A_1-560 with R522D mutation

    Depositor

    Jukka Westermarck

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R522D (522 AGA->GAT)

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T_CIP2A_1-560_L533E

    Plasmid

    #217924

    Purpose

    E. coli expression of GST-tagged CIP2A_1-560 with L533E mutation

    Depositor

    Jukka Westermarck

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L533E (533 TTG->GAG)

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MP-J

    Plasmid

    #69647

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    rpsD12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MP-J2

    Plasmid

    #69648

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    rpsD14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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