We narrowed to 1,719 results for: plasmid for e coli

1. pBEL1995

   Plasmid

   #139899

   Purpose

   Expression plasmid for LetB PLL3 deletion (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta347-360, P346G)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL1891

   Plasmid

   #139895

   Purpose

   Complementation plasmid for LetAB with LetB PLL6 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta703-708, Q702G, E709G)

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL1887

   Plasmid

   #139891

   Purpose

   Complementation plasmid for LetAB with LetB PLL2 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta229-239, D228G, L240G)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL1888

   Plasmid

   #139892

   Purpose

   Complementation plasmid for LetAB with LetB PLL3 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta347-360, P346G)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL1886

   Plasmid

   #139890

   Purpose

   Complementation plasmid for LetAB with LetB PLL1 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta114-128, P113G)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2009

   Plasmid

   #139884

   Purpose

   Complementation plasmid for LetAB with 6 ring (rings 123457) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta634-746)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL2008

   Plasmid

   #139883

   Purpose

   Complementation plasmid for LetAB with 5 ring (rings 12347) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta515-746)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL2007

   Plasmid

   #139882

   Purpose

   Complementation plasmid for LetAB with 4 ring (rings 1567) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta161-514)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL2006

   Plasmid

   #139881

   Purpose

   Complementation plasmid for LetAB with 3 ring (rings 167) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta161-634)

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL1755

    Plasmid

    #139879

    Purpose

    Expression plasmid for naturally occurring 8xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Denitrovibrio_acetiphilus_Dacet_0211
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Denitrovibrio_acetiphilus_Dacet_0211(41-988)-6xHis

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1753

    Plasmid

    #139878

    Purpose

    Expression plasmid for naturally occurring 6xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Pseudomonas_aeruginosa_PA4689
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas_aeruginosa_PA4689(41-768)-6xHis

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1752

    Plasmid

    #139877

    Purpose

    Expression plasmid for naturally occurring 5xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Nitrincola_nitratireducens_D791_00999
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nitrincola_nitratireducens_D791_00999(47-644)-6xH…

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL1751

    Plasmid

    #139876

    Purpose

    Expression plasmid for naturally occurring 4xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Desulfocapsa_sulfexigens_UWK_02604
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Desulfocapsa_sulfexigens_UWK_02604(38-533)-6xHis

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL1584

    Plasmid

    #139875

    Purpose

    Expression plasmid of rings 2 and 3 of LetB for crystallography

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(159-383)-6xHis

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJF6

    Plasmid

    #21678

    Depositor

    James Haber

    Article

    Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    HO digestion site in one of the LacZ genes

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pJF5

    Plasmid

    #21679

    Depositor

    James Haber

    Article

    Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    HO digestion site in one of the LacZ genes

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid

    #162576

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K mutated to give dominant negative phenotype

    Promoter

    pBAD

    Available Since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge