We narrowed to 23,643 results for: SHR;

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pX459-sg-CMV162

Plasmid

#179917

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EBFP_Nick_Dual_sgRNA

Plasmid

#178094

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EBFP nick sgRNAs

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EGFP_Nick_Dual_sgRNA

Plasmid

#178095

Purpose

Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EGFP nick sgRNAs

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFB_ROSA26_SpCas9_gRNAs

Plasmid

#174703

Purpose

Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

Depositor

Beverly L. Davidson

Article

Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

Insert

SpCas9 dual gRNAs

Use

AAV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Tpi1

Plasmid

#175940

Purpose

knockout mouse Tpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Tpi1 gRNA (Tpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Olfr2

Plasmid

#175935

Purpose

knockout mouse Olfr2

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Olfr2 gRNA (Or6a2 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-whiteCopyCatcher

Plasmid

#174062

Purpose

CopyCatcher insertion donor for the white locus

Depositor

Ethan Bier

Article

Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

Insert

white (w Synthetic, Fly)

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#1

Plasmid

#170834

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gLacZ.EFS-NS.H2B-RFP

Plasmid

#170363

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2hygro-EGFP-FF2

Plasmid

#164921

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK_1

Plasmid

#163462

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 1 targeting NADK (NADK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p8115 LentiCRISPR v2 sgPTPN14-3

Plasmid

#163314

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

sgPTPN14-3

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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