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pGEX6-Smad3 EPSM A213S

Plasmid

#27110

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A213S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pBOBI C-FLAG PHGDH

Plasmid

#212995

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Clathrin-mGreenLantern

Plasmid

#164462

Purpose

Localization of mGreenLantern fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Clathrin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-WT-V5

Plasmid

#167468

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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HRE-dUnaG

Plasmid

#201711

Purpose

Hypoxia responsive promoter driving destablized UnaG fluorescent protein

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSLHDR02_EGFP_tCD8

Plasmid

#124061

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

EGFP
tCD8

Use

Mouse Targeting; Human targeting

Promoter

Human UbC promoter modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Tags

6 His tag

Expression

Bacterial

Promoter

ARA promoter

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.1-c002

Plasmid

#173087

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N369, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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APLF-c001

Plasmid

#173077

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

APLF (APLF Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K360-V448, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TARG1-c002

Plasmid

#173108

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

TARG1

Tags

mCerulean

Expression

Bacterial

Mutation

Full length protein

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHdCas9

Plasmid

#137080

Purpose

Expression of Himar-dCas9 in bacteria

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Expression

Bacterial

Promoter

pLtetO-1

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRLCon1-3180

Plasmid

#31445

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (long) (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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HRE-dUnOHR

Plasmid

#201714

Purpose

Hypoxia responsive promoter driving UnaG and mOrange

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Tal1

Plasmid

#60670

Purpose

PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Tal1 (Tal1 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PB-Sox17

Plasmid

#60669

Purpose

PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Sox17 (Sox17 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CIRTS-control

Plasmid

#213788

Purpose

CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

Insert

CIRTS-Calm3-U6-control gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

Syn1, U6

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RR-ABE8e-V106W

Plasmid

#193656

Purpose

Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RVR-ABE8e-V106W

Plasmid

#193659

Purpose

Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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