We narrowed to 23,870 results for: pcDNA

Showing: 9841 - 9860 of 23870 results

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pcDNA3.1-SARS2-Spike-HiBiT

Plasmid

#191212

Purpose

Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SARS-CoV-2 spike_WT (S SARS CoV-2)

Tags

HiBiT (high affinity small nanoluciferase fragmen…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA8

Plasmid

#19541

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-AT1R-APEX

Plasmid

#96847

Purpose

For expression of AT1R-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

AT1R-APEX (AGTR1 Human)

Expression

Mammalian

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2CP-VPg(FCV)

Plasmid

#167314

Purpose

Template pDNA to prepare CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-isoform a

Plasmid

#225447

Purpose

Express EGFP-fusion protein of FXR1 isoform a

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1A

Plasmid

#19483

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1A (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA8

Plasmid

#19487

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA8 (HSPA8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-CIP2A

Plasmid

#222623

Purpose

mammalian expression vector of Flag-tagged CIP2A

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-eGFP-msMMS22L

Plasmid

#221014

Purpose

mammalian expression vector of eGFP tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 Lats1 (Nigg HS189)

Plasmid

#41156

Depositor

Erich Nigg

Article

Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

Insert

Lats1 (LATS1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-smURFP-IRES-eGFP

Plasmid

#80343

Purpose

Expresses smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
eGFP

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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