We narrowed to 59,942 results for: %s

1. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

   Plasmid

   #153524

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#16

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#16
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI syn iGluu

   Plasmid

   #106122

   Purpose

   Fast indicator for synaptic glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   synapsin

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP2A_NLS_sfGFP

   Plasmid

   #63709

   Purpose

   Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
   

   Use

   Just a donor dna with nanog homologous arm for kn…

   Tags

   sfGFP

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSIV-TRE-ASCL1

   Plasmid

   #154883

   Purpose

   Doxycycline-dependently expresses human ASCL1.

   Depositor

   Hideyuki Okano

   Article

   Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

   Insert

   ASCL1 (ASCL1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA_tdtomato_reporter1_SP-2x

   Plasmid

   #79370

   Purpose

   Assays activity of transcriptional activators fused to SP Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Bmpr2ΔKinase

   Plasmid

   #163404

   Purpose

   Expresses BMPR-2 lacking the kinase domain in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-N-CretrcintG

   Plasmid

   #69572

   Purpose

   One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

   Insert

   kozak consensus - N-CretrcintG
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-C-CreintG

    Plasmid

    #69573

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Insert

    kozak consensus - C-CreintG
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. eNme2-C TadDE

    Plasmid

    #193842

    Purpose

    Express TadDE (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB3K3 containing mRFP1

    Plasmid

    #118083

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SspB-HaloTag-DHPH-TIAM1

    Plasmid

    #176114

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-FKBP-GFP-SINTBAD

    Plasmid

    #216840

    Purpose

    Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB1K3 containing mRFP1

    Plasmid

    #118082

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHS1184

    Plasmid

    #205965

    Purpose

    NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH
    

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBT-GCaMP6-HA

    Plasmid

    #107433

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    GCaMP6S
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SspB-mCherry-DHPH-TIAM1

    Plasmid

    #176104

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge