We narrowed to 11,584 results for: Vars;

1. pJOG638

   Plasmid

   #105389

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor CaBs3 (1000bp), variant
   

   Use

   Synthetic Biology

   Mutation

   TALE-binding site; BsaI/ BpiI restriction sites r…

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJOG639

   Plasmid

   #105390

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor CaBs3 (1000bp), variant
   

   Use

   Synthetic Biology

   Mutation

   TALE-binding site; BsaI/ BpiI restriction sites r…

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOG142

   Plasmid

   #105397

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   mYFP (GFP variant) fused to PKI nuclear export signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJOG143

   Plasmid

   #105398

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   mYFP (GFP variant) fused to SV40 nuclear localization signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. P0050::kaiBC S431A T432A NS1

   Plasmid

   #101837

   Purpose

   drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431A T432A with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431A T432A

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. P0050::kaiBC S431E T432E NS1

   Plasmid

   #101836

   Purpose

   drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431E T432E with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431E T432E

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

   Plasmid

   #91945

   Purpose

   Bacterial expression of maize SAE1 and truncated SAE2

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   HA-ZmSAE1
   ZmSAE2-FLAG
   

   Tags

   FLAG and HA

   Expression

   Bacterial

   Mutation

   Truncation of ZmSAE2 to mimic a splice variant

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3R112C/T180C-GG

   Plasmid

   #85093

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1G108C-GG

   Plasmid

   #85092

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Glycine 108 to Cysteine

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-6NBC-GFP-PGK-Puro-Barcode9

    Plasmid

    #83686

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode9
    

    Use

    Retroviral

    Mutation

    please view depositor comments below

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-6NBC-GFP-PGK-Puro-Barcode7

    Plasmid

    #83684

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode7
    

    Use

    Retroviral

    Mutation

    L119P in GFP

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-KG/Net1A

    Plasmid

    #28261

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pSB700 Cerulean-Weissman scaffold

    Plasmid

    #79377

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700 Cerulean-Zhang2.0

    Plasmid

    #79378

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    zhang 2.0
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAF0687 Pa01_attP-mCherry donor

    Plasmid

    #193466

    Purpose

    Promoterless mcherry donor for Pa01

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAF0451 Si74_attP-mCherry donor

    Plasmid

    #193470

    Purpose

    Promoterless mcherry donor for Si74

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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