We narrowed to 14,905 results for: sta
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Plasmid#196517PurposeExpression of Ctdnep1WT-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCtdnep1WTsiR (CTDNEP1 Human)
TagseGFPExpressionMammalianMutationSilent mutations for siRNA resistancePromoterCMV promoter; T7 promoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 899
Plasmid#194193PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseMutationmutated KLF binding sites (position 899)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDC-DIO-GFP-shRNA.scramble
Plasmid#184331PurposeExpresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expressionDepositorInserthdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiExpressionMammalianPromoterhistidine decarboxylase promoter fragment that g…Available sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mILK-L207W-EFGP
Plasmid#176900Purposemouse ILK with L207W point mutation cloned into pEGFP-N1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIntegrin-linked kinase (Ilk Mouse)
TagsEGFPExpressionMammalianMutationLeucine 207 to TryptophanePromoterCNVAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/RBD-puro
Plasmid#161793PurposeTransposon-based SARS-CoV-2 RBD stable expression vector for protein productionDepositorInsertSARS-CoV-2 RBD
UseTransposonTags6xHis and dTomatoExpressionMammalianPromoterEF1a/RPBSAAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-GFP-EphrinB3-Flag(A227Y)
Plasmid#155013PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEphrinB3 (EPHB3 Human)
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA227YPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLE1 C-ISCmut
Plasmid#160804PurposeExpress C-terminal ISC mutant POLE1DepositorInsertPOLE1 C-ISCmut (POLE Human)
UseRetroviralTags3xFLAGMutationshPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…Available sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g1 (BB22)
Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPV00605 pET21GG2-His(6)-BECN(1-265)
Plasmid#136686PurposeExpresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 IDR + CC (BECN1 Human)
TagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MMP2AG-T2APuroR-5h
Plasmid#128256PurposeMammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrtTA-Advanced::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nanog-t2A-mCherry
Plasmid#127541PurposeExpresses the transactivation domain of mouse Nanog and mCherry via t2A linker.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog (Nanog Mouse)
ExpressionMammalianMutationCodes for the last 110 a.a of NanogPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL649 CHYRON2 integration construct
Plasmid#126446PurposeTo integrate the CHYRON2 locus at AAVS1 in human cells.DepositorInsertspU6-CHYRON2 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q769gp120-8his
Plasmid#100929PurposeMammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strainDepositorInsertHIV-1 (Q769.d22) Env
TagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-D882N
Plasmid#114062Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBUBR1-D882N (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-D882NAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795R
Plasmid#114060Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBUBR1-K795R (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795RAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-MIS12-KARD
Plasmid#114057Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMIS12-KARD (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(31-114)-9myc
Plasmid#99517PurposeTruncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiaeDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
DPAGT1 (DPAGT1A-c005)
Plasmid#98228PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPAGT1A (DPAGT1 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00953:Glycos_transf_4 98-275PromoterpolyhedrinAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only