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pAC-PHYTipi

Plasmid

#53283

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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PMXS-OST4-mScarlet-3xHA-IRES-blasticidin

Plasmid

#255991

Purpose

PMXS construct expressing OST4-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

OST4-mScarlet-3xHA (OST4 Human)

Use

Retroviral

Tags

mScarlet-3xHA

Expression

Mammalian

Mutation

N/A

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xMYC-IRES-bleomycin

Plasmid

#255988

Purpose

PMXS construct expressing EMC3-mScarlet-3xMYC with an IRES-bleomycin cassette for use as an IP control

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xMYC (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xMYC

Expression

Mammalian

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-OST4-mScarlet-3xMYC-IRES-blasticidin

Plasmid

#255992

Purpose

PMXS construct expressing OST4-mScarlet-3xMYC with an IRES-blasticidin cassette for use as an IP control.

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

OST4-mScarlet-3xMYC (OST4 Human)

Use

Retroviral

Tags

mScarlet-3xMYC

Expression

Mammalian

Mutation

N/A

Available Since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xHA-IRES-blasticidin

Plasmid

#255989

Purpose

PMXS construct expressing EMC3-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xHA (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xHA

Expression

Mammalian

Available Since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xHA-IRES-bleomycin

Plasmid

#255987

Purpose

PMXS construct expressing EMC3-mScarlet-3xHA with an IRES-bleomycin cassette for ER-IP

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xHA (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xHA

Expression

Mammalian

Mutation

N/A

Available Since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xMYC-IRES-blasticidin

Plasmid

#255990

Purpose

PMXS construct expressing EMC3-mScarlet-3xMYC with an IRES-blasticidin cassette for use as an IP control.

Depositor

Kivanc Birsoy

Article

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xMYC (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xMYC

Expression

Mammalian

Mutation

N/A

Available Since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ512-pAAV-PB3'IR-CAG-MCP-mtagBFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254952

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ514-pAAV-PB3'IR-CAG-PCP-mScarlet-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254953

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ515-pAAV-PB3'IR-CAG-PCP-mtagBFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254954

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR

Plasmid

#254955

Purpose

AAV piggyBac vector with single gRNA and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ521-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-hU6-gRNA-PB5'IR

Plasmid

#254956

Purpose

AAV piggyBac vector with single gRNA and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ494-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254949

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-GFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ496-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254950

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ511-pAAV-PB3'IR-CAG-MCP-mScarlet-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254951

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2278

Plasmid

#252579

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA4 and AtPDS3 gRNA12

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA 4 and AtPDS3 gRNA12

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2279

Plasmid

#252580

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtPDS3 gRNA 12 and AtCHLl1 gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2498

Plasmid

#252581

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA6 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA6 and gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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