We narrowed to 15,730 results for: NOL

1. pLCHKO_HPRT1_exon2_2_Lb

   Plasmid

   #155052

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2-Snrnp40

   Plasmid

   #134255

   Purpose

   Lentivector for Snrnp40 CRISPR knockout

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   CRISPR and Lentiviral

   Mutation

   Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

   Promoter

   U6

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOB-N-Flag-Snrnp40

   Plasmid

   #134249

   Purpose

   Lentivector encoding Flag-tagged Snrnp40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Met133I, M1381I, M186I ELL2

   Plasmid

   #127263

   Purpose

   For in vitro translation of human ELL2 with Met133I, M1381I, M186I

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Three Mets (133, 138, 186) to Ileu

   Promoter

   T7

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 563aa ELL2 pEF4B

   Plasmid

   #127273

   Purpose

   Expresses 563aa human WT ELL2

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 truncated at SphI (ELL2 Human)
   

   Expression

   Mammalian

   Mutation

   Contains 564 N-terminal amino acids ELL2, termina…

   Promoter

   pEF1a

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKdH_P-GAP-ScACS1*

   Plasmid

   #126736

   Purpose

   yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, P113S, and S629N (please see dep…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

   Plasmid

   #126738

   Purpose

   yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2,acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C

   Promoter

   GAP

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA tagged Sph ELL2

   Plasmid

   #127270

   Purpose

   For in vitro translation of human WT ELL2 with internal HA tag

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   WT Mets and HA tag at SphI site (8th Met)

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA tagged mid ELL2

   Plasmid

   #127274

   Purpose

   Expresses human ELL2 with HA tag in the middle that does not disrupt function

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with HA at XbaI (ELL2 Human)
   

   Expression

   Mammalian

   Mutation

   HA inserted at XbaI after Met 186

   Promoter

   pEF1a

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Met138ILeu ELL2

    Plasmid

    #127260

    Purpose

    For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met138 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Met186ILeu ELL2

    Plasmid

    #127261

    Purpose

    For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met186 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met186 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Met138ILeu, Met186ILeu ELL2

    Plasmid

    #127265

    Purpose

    For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Two Mets (138,186) to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Met133ILeu ELL2

    Plasmid

    #127259

    Purpose

    For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met133 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Met133ILeu, Met186ILeu ELL2

    Plasmid

    #127264

    Purpose

    For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Two Mets (133,186 )to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Met331Leu ELL2

    Plasmid

    #127262

    Purpose

    For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met331 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met331 to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. COOHend ELL2

    Plasmid

    #127255

    Purpose

    Expresses the C terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (C-terminal end) (ELL2 Human)
    

    Use

    Other

    Expression

    Mammalian

    Mutation

    contains only the last 389 aa

    Promoter

    pEF1a

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Met1ILeu ELL2

    Plasmid

    #127258

    Purpose

    For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met1 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met1 to Ileu by Quikchange

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. ELL2mRNA+polyA

    Plasmid

    #127256

    Purpose

    For in vitro translation of human ELL2 with 30 nt pA tail

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    shortens 3'UTR to 40 bp adds A30 tail

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NH2end ELL2

    Plasmid

    #127252

    Purpose

    Expresses the N terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (1-287) (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    contains only amino acids 1-289

    Promoter

    pEF1a

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only